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mirna expression profiling microarray (Agilent technologies)

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Agilent technologies mirna expression profiling microarray
Mirna Expression Profiling Microarray, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mirna+expression+profile+microarray/pm37461104-100-12-16
Average 90 stars, based on 1 article reviews

mirna expression profiling microarray - by Bioz Stars, 2026-11

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Article Title: Distinct microRNA expression profiles in follicle-associated epithelium and villous epithelium
Article Snippet: Total RNA was extracted from isolated FAE and VE using mirVana kit (Ambion) according to the manufacturer's instructions. miRNA expression profile was assessed by using Mouse miRNA Microarray Release 12.0 (Agilent Technologies).

Article Title: miR-204 regulates epithelial-mesenchymal transition by targeting SP1 in the tubular epithelial cells after acute kidney injury induced by ischemia-reperfusion.
Article Snippet: To explore the molecular mechanism how I/R injury altered the miRNA expression, we assessed the miRNA profile by means of a novel micro-bead based technology Agilent Mouse miRNA Microarray Scanner formats (8x60 K) which contain probes for 1881 mouse mature miRNAs from the Sanger miRBase V21.0.

Article Title: Bioinformatic screening for candidate biomarkers and their prognostic values in endometrial cancer
Article Snippet: In this dataset, the miRNA expression profile was detected using the GPL7731 Agilent-019118 Human miRNA Microarray 2.0 G4470B platform.

Article Title: SHOC2 scaffold protein modulates daunorubicin-induced cell death through p53 modulation in lymphoid leukemia cells
Article Snippet: MicroRNA library was obtained from total RNA (100ηg) and the expression profile was performed using the Agilent “Human micro-RNA Microarray Kit (v3)” (G4470C, Agilent Technologies, Santa Clara, CA, USA) as previously reported .

Article Title: Implication of microRNA regulation in para -phenylenediamine-induced cell death and senescence in normal human hair dermal papilla cells
Article Snippet: Following 24 h of treatment, total RNA was purified using TRIzol reagent (Life Technologies) according to the manufacturer’s instructions.

Expressing:

Article Title: MicroRNA-505, Suppressed by Oncogenic Long Non-coding RNA LINC01448, Acts as a Novel Suppressor of Glycolysis and Tumor Progression Through Inhibiting HK2 Expression in Pancreatic Cancer
Article Snippet: The total RNA from five PC tissues and paired normal tissues was isolated using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA). .. The expression profile of miRNAs was analyzed using the Agilent Human miRNA expression array (8 × 60K platform, Agilent, Inc., Santa Clara, CA, USA) following the manufacturer's instructions. .. In brief, 100 ng of total RNA from each specimen was labeled with cyanine 3-pCp (Cy3) using the miRNA Complete Labeling and Hyb kit (Agilent).

Article Title: Identification of key miRNA-gene pairs in chronic lymphocytic leukemia through integrated analysis of mRNA and miRNA microarray
Article Snippet: .. MiRNA expression profile was detected via the Agilent-021827 Human miRNA Microarray G4470C platform. ..

Article Title: Inflammation-induced endothelial cell-derived extracellular vesicles modulate the cellular status of pericytes
Article Snippet: Primer sequences are available upon request from the TAKARA BIO INC. website ( http://www.takara-bio.co.jp/ ). .. The miRNA expression profile was obtained by using a Mouse miRNA microarray 8 × 15 K (Agilent Technologies, Santa Clara, CA) according to the manufacturer's protocol. .. Total RNA was prepared from fractionated E-EVs using the miRNA easy Mini Kit (Qiagen).

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Study design flowchart. circRNA, circular RNA; miRNA, microRNA; RIF, recurrent implantation failure. DEcircRNAs, differentially expressed circRNAs; DEmiRNAs, differentially expressed miRNAs; DEmRNAs, differentially expressed mRNAs

Journal: BMC Medical Genomics

Article Title: Comprehensive analysis of circRNA-miRNA-mRNA network related to angiogenesis in recurrent implantation failure

doi: 10.1186/s12920-024-01944-1

Figure Lengend Snippet: Study design flowchart. circRNA, circular RNA; miRNA, microRNA; RIF, recurrent implantation failure. DEcircRNAs, differentially expressed circRNAs; DEmiRNAs, differentially expressed miRNAs; DEmRNAs, differentially expressed mRNAs

Article Snippet: CircRNA, miRNA and mRNA microarray expression profile datasets were selected from the National Center of Biotechnology Information Gene Expression Omnibus (NCBI) GEO ( https://www.ncbi.nlm.nih.gov/geo/ ).

Techniques:

Boxplots, volcano plots and Venn diagram of mRNAs for GSE103465. (A) Boxplot of GSE103465. (B) Volcano plots of DEmRNAs based on GSE103465. (C) Venn diagram of mRNAs for GeneCards and GSE103465, where the intersection section is predicted DEmRNAs asscociated with angiogenesis in RIF. circRNA, circular RNA; miRNA, microRNA; RIF, recurrent implantation failure

Journal: BMC Medical Genomics

Article Title: Comprehensive analysis of circRNA-miRNA-mRNA network related to angiogenesis in recurrent implantation failure

doi: 10.1186/s12920-024-01944-1

Figure Lengend Snippet: Boxplots, volcano plots and Venn diagram of mRNAs for GSE103465. (A) Boxplot of GSE103465. (B) Volcano plots of DEmRNAs based on GSE103465. (C) Venn diagram of mRNAs for GeneCards and GSE103465, where the intersection section is predicted DEmRNAs asscociated with angiogenesis in RIF. circRNA, circular RNA; miRNA, microRNA; RIF, recurrent implantation failure

Techniques:

Boxplots, volcano plots and Venn diagram of miRNAs for GSE121219. (A) Boxplot of GSE121219. (B) Volcano plots of DEmiRNAs based on GSE121219. (C) Venn diagram of miRNAs for miRDIP and GSE121219, where the intersection section is predicted DEmiRNAs asscociated with angiogenesis in RIF. circRNA, circular RNA; miRNA, microRNA; RIF, recurrent implantation failure

Journal: BMC Medical Genomics

Article Title: Comprehensive analysis of circRNA-miRNA-mRNA network related to angiogenesis in recurrent implantation failure

doi: 10.1186/s12920-024-01944-1

Figure Lengend Snippet: Boxplots, volcano plots and Venn diagram of miRNAs for GSE121219. (A) Boxplot of GSE121219. (B) Volcano plots of DEmiRNAs based on GSE121219. (C) Venn diagram of miRNAs for miRDIP and GSE121219, where the intersection section is predicted DEmiRNAs asscociated with angiogenesis in RIF. circRNA, circular RNA; miRNA, microRNA; RIF, recurrent implantation failure

Techniques:

Boxplots, volcano plots and Venn diagram of circRNAs for GSE147442. (A) Boxplot of GSE147442 after standardization. (B) Volcano plots of DEcircRNAs based on GSE147442. (C) Venn diagram of miRNAs for starBase 2.0 and GSE147442, where the intersection section is predicted DEcircRNAs asscociated with angiogenesis in RIF. circRNA, circular RNA; miRNA, microRNA; RIF, recurrent implantation failure

Journal: BMC Medical Genomics

Article Title: Comprehensive analysis of circRNA-miRNA-mRNA network related to angiogenesis in recurrent implantation failure

doi: 10.1186/s12920-024-01944-1

Figure Lengend Snippet: Boxplots, volcano plots and Venn diagram of circRNAs for GSE147442. (A) Boxplot of GSE147442 after standardization. (B) Volcano plots of DEcircRNAs based on GSE147442. (C) Venn diagram of miRNAs for starBase 2.0 and GSE147442, where the intersection section is predicted DEcircRNAs asscociated with angiogenesis in RIF. circRNA, circular RNA; miRNA, microRNA; RIF, recurrent implantation failure

Techniques:

Figure 5 CircRNA-miRNA-mRNA regulatory network, which consists of 8 DEcircRNAs, 10 DEmiRNAs and 45 DERNAs. DEcircRNA, differentially expressed circular RNA; DEmiRNA, differentially expressed micro RNA; DERNA, differentially expressed RNA. Arrow-shape nodes: circRNAs, triangle nodes: miRNAs, ellipse-shaped nodes: mRNAs

Journal: BMC Medical Genomics

Article Title: Comprehensive analysis of circRNA-miRNA-mRNA network related to angiogenesis in recurrent implantation failure

doi: 10.1186/s12920-024-01944-1

Figure Lengend Snippet: Figure 5 CircRNA-miRNA-mRNA regulatory network, which consists of 8 DEcircRNAs, 10 DEmiRNAs and 45 DERNAs. DEcircRNA, differentially expressed circular RNA; DEmiRNA, differentially expressed micro RNA; DERNA, differentially expressed RNA. Arrow-shape nodes: circRNAs, triangle nodes: miRNAs, ellipse-shaped nodes: mRNAs

Techniques:

A PPI network and circRNA–miRNA–hub gene regulatory subnetwork. (A) A PPI network of the fifty-five target genes associated with angiogenesis in RIF. (B) Six hub genes extracted by cytoHubba plug-in. (C) CircRNA–miRNA–hub gene regulatory subnetwork, consisting of 3 circRNAs, 3 miRNAs, and 3 mRNAs. PPI, protein–protein interaction; circRNA, circular RNA; miRNA, microRNA; RIF, recurrent implantation failure

Journal: BMC Medical Genomics

Article Title: Comprehensive analysis of circRNA-miRNA-mRNA network related to angiogenesis in recurrent implantation failure

doi: 10.1186/s12920-024-01944-1

Figure Lengend Snippet: A PPI network and circRNA–miRNA–hub gene regulatory subnetwork. (A) A PPI network of the fifty-five target genes associated with angiogenesis in RIF. (B) Six hub genes extracted by cytoHubba plug-in. (C) CircRNA–miRNA–hub gene regulatory subnetwork, consisting of 3 circRNAs, 3 miRNAs, and 3 mRNAs. PPI, protein–protein interaction; circRNA, circular RNA; miRNA, microRNA; RIF, recurrent implantation failure

Techniques:

Hematoxylin-eosin staining of control, ECRSwNP, and non-ECRSwNP groups, and differential expression of circRNAs and miRNAs in the three groups. (A) Representative images of hematoxylin-eosin staining. The scale bar represents 100 μm. (B) Hierarchical clustering analysis heat map showing significantly changed of circRNAs and (C) of miRNAs with fold change ≥2.0 ( p < 0.05) in the three groups (ECRSwNP, non-ECRSwNP, and control). The expressions of circRNAs and miRNAs were significantly different between CRSwNP and control groups, but ECRSwNP and non-ECRSwNP groups shared similar gene expression profiles. Then, they were combined as the CRSwNP group. Hierarchical clustering analysis heat map showing significantly changed of circRNAs (D) and of miRNAs (E) with fold change ≥2.0 ( p < 0.05) in the two groups (CRSwNP and control).

Journal: Frontiers in Molecular Biosciences

Article Title: Gene Expression Profiles of Circular RNAs and MicroRNAs in Chronic Rhinosinusitis With Nasal Polyps

doi: 10.3389/fmolb.2021.643504

Figure Lengend Snippet: Hematoxylin-eosin staining of control, ECRSwNP, and non-ECRSwNP groups, and differential expression of circRNAs and miRNAs in the three groups. (A) Representative images of hematoxylin-eosin staining. The scale bar represents 100 μm. (B) Hierarchical clustering analysis heat map showing significantly changed of circRNAs and (C) of miRNAs with fold change ≥2.0 ( p < 0.05) in the three groups (ECRSwNP, non-ECRSwNP, and control). The expressions of circRNAs and miRNAs were significantly different between CRSwNP and control groups, but ECRSwNP and non-ECRSwNP groups shared similar gene expression profiles. Then, they were combined as the CRSwNP group. Hierarchical clustering analysis heat map showing significantly changed of circRNAs (D) and of miRNAs (E) with fold change ≥2.0 ( p < 0.05) in the two groups (CRSwNP and control).

Article Snippet: MiRNA expression profile microarrays of these specimens were performed by CapitalBio.

Techniques: Staining, Control, Quantitative Proteomics, Gene Expression

Different expressions of circRNAs and miRNAs shown in volcano plots. The circRNAs and miRNAs were widely distributed in all chromosomes. Each column corresponds to a circRNA or a miRNA, the green column toward the center represents significantly downregulated and the red one toward the outside means upregulated. (A) Differential expression of circRNAs. (B) Differential expression of miRNAs.

Journal: Frontiers in Molecular Biosciences

Article Title: Gene Expression Profiles of Circular RNAs and MicroRNAs in Chronic Rhinosinusitis With Nasal Polyps

doi: 10.3389/fmolb.2021.643504

Figure Lengend Snippet: Different expressions of circRNAs and miRNAs shown in volcano plots. The circRNAs and miRNAs were widely distributed in all chromosomes. Each column corresponds to a circRNA or a miRNA, the green column toward the center represents significantly downregulated and the red one toward the outside means upregulated. (A) Differential expression of circRNAs. (B) Differential expression of miRNAs.

Article Snippet: MiRNA expression profile microarrays of these specimens were performed by CapitalBio.

Techniques: Quantitative Proteomics

Top 40 circRNA-miRNA co-expression networks. Co-expression networks were analyzed by miRanda-3.3 software, combined with entropy values below 20. Stars represent circRNAs; squares represent miRNA; lines represent correlative relationships. Different sizes and colors represent the corresponding (up/down) relationship.

Journal: Frontiers in Molecular Biosciences

Article Title: Gene Expression Profiles of Circular RNAs and MicroRNAs in Chronic Rhinosinusitis With Nasal Polyps

doi: 10.3389/fmolb.2021.643504

Figure Lengend Snippet: Top 40 circRNA-miRNA co-expression networks. Co-expression networks were analyzed by miRanda-3.3 software, combined with entropy values below 20. Stars represent circRNAs; squares represent miRNA; lines represent correlative relationships. Different sizes and colors represent the corresponding (up/down) relationship.

Article Snippet: MiRNA expression profile microarrays of these specimens were performed by CapitalBio.

Techniques: Expressing, Software

Top 10 significant enriched GO terms analysis of miRNAs. Differentially expressed miRNAs were mainly involved in biological process, cellular component, and molecular function. Compared with the normal subjects, the significantly over-presented miRNAs in the CRSwNP group were mainly involved in positive regulation of biological process, intracellular part, and binding.

Journal: Frontiers in Molecular Biosciences

Article Title: Gene Expression Profiles of Circular RNAs and MicroRNAs in Chronic Rhinosinusitis With Nasal Polyps

doi: 10.3389/fmolb.2021.643504

Figure Lengend Snippet: Top 10 significant enriched GO terms analysis of miRNAs. Differentially expressed miRNAs were mainly involved in biological process, cellular component, and molecular function. Compared with the normal subjects, the significantly over-presented miRNAs in the CRSwNP group were mainly involved in positive regulation of biological process, intracellular part, and binding.

Article Snippet: MiRNA expression profile microarrays of these specimens were performed by CapitalBio.

Techniques: Binding Assay

KEGG pathway analysis of circRNAs and miRNAs. Biological roles of the differentially expressed circRNAs (A) and miRNAs (B) . Biological roles of the differentially expressed circRNAs include amebiasis, salivary secretion, cell adhesion molecules (CAMs), and so on. Biological roles of the differentially expressed miRNAs include pathways in cancer, endocytosis, thyroid hormone signaling pathway, and so on.

Journal: Frontiers in Molecular Biosciences

Article Title: Gene Expression Profiles of Circular RNAs and MicroRNAs in Chronic Rhinosinusitis With Nasal Polyps

doi: 10.3389/fmolb.2021.643504

Figure Lengend Snippet: KEGG pathway analysis of circRNAs and miRNAs. Biological roles of the differentially expressed circRNAs (A) and miRNAs (B) . Biological roles of the differentially expressed circRNAs include amebiasis, salivary secretion, cell adhesion molecules (CAMs), and so on. Biological roles of the differentially expressed miRNAs include pathways in cancer, endocytosis, thyroid hormone signaling pathway, and so on.

Article Snippet: MiRNA expression profile microarrays of these specimens were performed by CapitalBio.

Techniques:

Confirmation of the expression of circRNAs and miRNAs using RNA-sequencing and quantitative real-time polymerase chain reaction (qRT-PCR). CircRNAs and miRNAs were validated by RNA-sequencing (CRSwNP( n = 6); control subjects ( n = 3) (A) ; and qRT-PCR in an independent cohort of five CRSwNP subjects and five control subjects (B) . Each sample was detected in triplicate. GAPDH was used as the reference gene for circRNAs, and hsa-miR-16 was used as the reference gene for miRNAs. The relative expression levels of hsa-circ-0031593, hsa-circ-0031594, hsa-circ-0109623, hsa-circ-0000736, hsa-miR-132-3p, hsa-miR-145-5p, hsa-miR-146a-5p, hsa-miR-125a-5p, and hsa-miR-27b-3p by qRT-PCR. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: Frontiers in Molecular Biosciences

Article Title: Gene Expression Profiles of Circular RNAs and MicroRNAs in Chronic Rhinosinusitis With Nasal Polyps

doi: 10.3389/fmolb.2021.643504

Figure Lengend Snippet: Confirmation of the expression of circRNAs and miRNAs using RNA-sequencing and quantitative real-time polymerase chain reaction (qRT-PCR). CircRNAs and miRNAs were validated by RNA-sequencing (CRSwNP( n = 6); control subjects ( n = 3) (A) ; and qRT-PCR in an independent cohort of five CRSwNP subjects and five control subjects (B) . Each sample was detected in triplicate. GAPDH was used as the reference gene for circRNAs, and hsa-miR-16 was used as the reference gene for miRNAs. The relative expression levels of hsa-circ-0031593, hsa-circ-0031594, hsa-circ-0109623, hsa-circ-0000736, hsa-miR-132-3p, hsa-miR-145-5p, hsa-miR-146a-5p, hsa-miR-125a-5p, and hsa-miR-27b-3p by qRT-PCR. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: MiRNA expression profile microarrays of these specimens were performed by CapitalBio.

Techniques: Expressing, RNA Sequencing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Control

Confirmation of the expression of circRNAs and miRNAs using quantitative real-time polymerase chain reaction (qRT-PCR). Sample numbers were expanded to perform qRT-PCR (CRSwNP ( n = 29); control subjects ( n = 25). GAPDH was used as the reference gene for circRNAs, and hsa-miR-16 was used as the reference gene for miRNAs. The heights of columns represent the fold changes of CRSwNP compared with the control group. The relative expression levels of hsa-circ-0031593, hsa-circ-0031594, hsa-circ-0109623, hsa-circ-0000736, hsa-miR-132-3p, hsa-miR-145-5p, hsa-miR-146a-5p, hsa-miR-125a-5p and hsa-miR-27b-3p by qRT-PCR. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: Frontiers in Molecular Biosciences

Article Title: Gene Expression Profiles of Circular RNAs and MicroRNAs in Chronic Rhinosinusitis With Nasal Polyps

doi: 10.3389/fmolb.2021.643504

Figure Lengend Snippet: Confirmation of the expression of circRNAs and miRNAs using quantitative real-time polymerase chain reaction (qRT-PCR). Sample numbers were expanded to perform qRT-PCR (CRSwNP ( n = 29); control subjects ( n = 25). GAPDH was used as the reference gene for circRNAs, and hsa-miR-16 was used as the reference gene for miRNAs. The heights of columns represent the fold changes of CRSwNP compared with the control group. The relative expression levels of hsa-circ-0031593, hsa-circ-0031594, hsa-circ-0109623, hsa-circ-0000736, hsa-miR-132-3p, hsa-miR-145-5p, hsa-miR-146a-5p, hsa-miR-125a-5p and hsa-miR-27b-3p by qRT-PCR. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: MiRNA expression profile microarrays of these specimens were performed by CapitalBio.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Control

Functional value of circRNAs and miRNAs. The receiver operating characteristic (ROC) curve analysis for the function of CRSwNP. The ROC curve analysis of hsa-circ-0031593, hsa-circ-0031594, hsa-miR-132-3p, hsa-miR-145-5p, hsa-miR-146a-5p, and hsa-miR-27b-3p and principal component 1 of these six ncRNAs for the significance in the occurrence and development of CRSwNP. The AUC (area under curves) values are given on the graphs.

Journal: Frontiers in Molecular Biosciences

Article Title: Gene Expression Profiles of Circular RNAs and MicroRNAs in Chronic Rhinosinusitis With Nasal Polyps

doi: 10.3389/fmolb.2021.643504

Figure Lengend Snippet: Functional value of circRNAs and miRNAs. The receiver operating characteristic (ROC) curve analysis for the function of CRSwNP. The ROC curve analysis of hsa-circ-0031593, hsa-circ-0031594, hsa-miR-132-3p, hsa-miR-145-5p, hsa-miR-146a-5p, and hsa-miR-27b-3p and principal component 1 of these six ncRNAs for the significance in the occurrence and development of CRSwNP. The AUC (area under curves) values are given on the graphs.

Article Snippet: MiRNA expression profile microarrays of these specimens were performed by CapitalBio.

Techniques: Functional Assay

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